Journal: Cell

Article Title: Single-Cell Analysis of Crohn’s Disease Lesions Identifies a Pathogenic Cellular Module Associated with Resistance to Anti-TNF Therapy

doi: 10.1016/j.cell.2019.08.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Primary antibodies were: anti-human CD3 (clone 2GV6, Ventana), anti-human CD20 (clone L26, Dako), anti-human DC-LAMP (clone 1010E1.01, Novus biologicals), anti-human CD68 (clone KP1, Dako), anti-human HLA-DR (clone TAL1B5, Abcam), anti-Ki67 (clone 30–09, Ventana), anti-human CD206 (polyclonal, Abcam) anti-PDPN (clone D2–40, Ventana).

Techniques: Recombinant, Multiplex Assay, Software

Journal: Kidney international

Article Title: Increased influx of myeloid dendritic cells during acute rejection is associated with interstitial fibrosis and tubular atrophy and predicts poor outcome.

doi: 10.1038/ki.2011.289

Figure Lengend Snippet: Figure 1 | Myeloid, plasmacytoid, and mature dendritic cells (DCs) are increased in rejection biopsies. Cryosections of (a–d) pretransplant and (e–h) rejection tissues were stained immunohistochemically for (a, e) BDCA-1, (b, f) DC-SIGN, (c, g) BDCA-2, and (d, h) DC-LAMP as described in the Materials and Methods. Original magnification 100. BDCA-1, blood dendritic cell antigen 1; BDCA-2, blood dendritic cell antigen 2; DC-LAMP, DC-lysosomal-associated membrane protein; DC-SIGN, DC-specific intercellular adhesion molecule-3-grabbing nonintegrin.

Article Snippet: After slides were fixed in ice-cold acetone, endogenous peroxidases were blocked with 0.1% NaN3 and 0.1% H2O2 in phosphate-buffered saline for 30 min, blocked for 45 min with 1% bovine serum albumin and 5% normal human serum in phosphate-buffered saline, and subsequently incubated overnight with the following primary antibodies, all of mouse origin: BDCA-1, BDCA-2 (Miltenyi Biotec, Utrecht, The Netherlands); DC-SIGN (R&D Systems, Abingdon, UK); and DC-LAMP (Immunotech, Marseille, France).

Techniques: Staining, Membrane

Journal: Kidney international

Article Title: Increased influx of myeloid dendritic cells during acute rejection is associated with interstitial fibrosis and tubular atrophy and predicts poor outcome.

doi: 10.1038/ki.2011.289

Figure Lengend Snippet: Figure 3 | Increase of dendritic cells (DCs) in pretransplant vs. rejection tissue. The presence of cells positive for BDCA-1, DC- SIGN, BDCA-2, and DC-LAMP was quantified for all coupled rejection and pretransplant biopsies. Single dots represent the fold increase for each rejection–pretransplant pair. Horizontal lines represent the median fold increase. BDCA-1, blood dendritic cell antigen 1; BDCA-2, blood dendritic cell antigen 2; DC-LAMP, DC-lysosomal-associated membrane protein; DC-SIGN, DC-specific intercellular adhesion molecule-3-grabbing nonintegrin.

Article Snippet: After slides were fixed in ice-cold acetone, endogenous peroxidases were blocked with 0.1% NaN3 and 0.1% H2O2 in phosphate-buffered saline for 30 min, blocked for 45 min with 1% bovine serum albumin and 5% normal human serum in phosphate-buffered saline, and subsequently incubated overnight with the following primary antibodies, all of mouse origin: BDCA-1, BDCA-2 (Miltenyi Biotec, Utrecht, The Netherlands); DC-SIGN (R&D Systems, Abingdon, UK); and DC-LAMP (Immunotech, Marseille, France).

Techniques: Membrane